| Club | Venue | Directions |
|---|---|---|
| Abingdon Utd Development Women | Northcourt Road | View Directions |
| Aldershot Town U19 | The EBB Stadium | View Directions |
| Amersham Town | Spratleys Meadow | View Directions |
| Ascot United Ladies Reserves | Ascot Racecourse | View Directions |
| Aveley | Parkside | View Directions |
| Bath City | Twerton Park | View Directions |
| Beaconsfield Town Womens 1st Team | Holloways Park | View Directions |
| Boreham Wood | Mangata Pay UK Stadium Meadow Park | View Directions |
| Boreham Wood U19 | Meadow Park | View Directions |
| Bristol Manor Farm | The Creek | View Directions |
| Caversham United Women | Scours Lane | View Directions |
| Chatham Town | Bauvill Stadium | View Directions |
| Chelmsford City | Melbourne Stadium | View Directions |
| Chertsey Town | Alwyns Lane | View Directions |
| Chesham United | The Meadow | View Directions |
| Chesham United Development | Chiltern Hills Academy | View Directions |
| Chichester City | Oaklands Park | View Directions |
| Chippenham Town | The Thornbury Surfacing Stadium | View Directions |
| Dartford FC U19 Academy | View Directions | |
| Dorking Wanderers | Meadowbank | View Directions |
| Dorking Wanderers U19 | Meadowbank | View Directions |
| Dover Athletic U19 | Crabble Athletic Ground | View Directions |
| Eastbourne Borough | The SO Legal Community Stadium | View Directions |
| Eastleigh FC U19 | Silverlake Stadium | View Directions |
| Enfield Town | Queen Elizabeth II Stadium | View Directions |
| Farnborough | BR Saunders Transport Community Stadium | View Directions |
| Hampton & Richmond | The Beveree Stadium | View Directions |
| Hanwell Town | The Powerday Stadium | View Directions |
| Hemel Hempstead Town | The Focus Community Arena | View Directions |
| Hornchurch | The Stadium | View Directions |
| Hungerford Town | Bulpit Lane | View Directions |
| Kidderminster Harriers | Aggborough Stadium | View Directions |
| Kidlington Youth | Exeter Park | View Directions |
| Maidenhead United CT U19 | Berkshire College of Agriculture | View Directions |
| Maidstone United | Gallagher Stadium | View Directions |
| Maidstone United U19 Academy | The Gallagher Stadium | View Directions |
| Milton United Ladies | Potash Lane | View Directions |
| Oxford City FC National League | Marsh Lane | View Directions |
| Penn & Tylers Green Ladies | French School Meadows | View Directions |
| QK Southampton | The Hive | View Directions |
| Salisbury | Raymond McEnhill Stadium | View Directions |
| Southend United Community Sports Club | Southend United FC Training Ground | View Directions |
| St Albans City | Clarence Park | View Directions |
| Tilehurst Panthers LFC | Theale Green School | View Directions |
| Tonbridge Angels | The Halcyon Wealth Longmead Stadium | View Directions |
| Torquay United | Plainmoor | View Directions |
| Truro City | Stadium for Cornwall | View Directions |
| Welling United | Park View Road | View Directions |
| Welling United U19 | Kidbrooke Playing Fields | View Directions |
| Weston-super-Mare | Optima Stadium | View Directions |
| Weymouth | Bob Lucas Stadium | View Directions |
| Woking | The Laithwaite Community Stadium | View Directions |
| Woking U19 | Westfield FC | View Directions |
| Worthing | Crucial Environmental Stadium | View Directions |
| Wycombe Wanderers u23 | The Gore | View Directions |