In a closely-contested game, which was a good work-out for both sides, Slough included former Chesham United midfielder Dom Marsala in their line-up. Marsala is a player who will be familiar to many Rebels fans, having scored the opening goal for the Generals in the 4-0 playoff final defeat at Chesham in May 2010.
Alex Brown pounced to volley home in the first half after a free-kick on the left flank was not cleared properly by the Holyport defence.
Although neither goalkeeper was tested too often, Jackson had to be alert to make a low save from Holyport's number 11 in the first half, and Danny Burnell missed the target on a couple of occasions after getting into good positions.
Trialists Baboucarr and Charlie Mpi, who has played for Thatcham Town, Maidenhead United and Wealdstone amongst others, also got run-outs, with under 18s regular Billy Canavan an unused substitute.
Slough now look forward to a first ever meeting with the new Windsor FC at Stag Meadow on Saturday.
Slough Town Line up
Jackson, Murphy, Woozley (c), Brown, Sturgess, Coyne, Logie (Coyle), Byrne (Baboucarr), Marsala (Mpi), Burnell, Sonner (Abbey). Subs: Fanner, Abbey, Coyle, Eales, Mpi, Baboucarr, Canavan.